Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 11 2 2012 06 15 165 173 EN Majid Mahmoodi Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran Ali Mohammad Alizadeh Cancer Research Center, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran Hamid Sohanaki Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Nima Rezaei Department of Immunology, Molecular Immunology Research Center, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Fatemeh Amini-Najafi Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Ali Reza Khosravi Department of Medical Parastiology, Mycology, School of Medicine, Tehran University, Tehran, Iran Sayed-Kazem Hosseini Tissue Bank Research &amp; Preparation Center, Tehran University of Medical Sciences, Tehran, Iran Zahra Safari Cancer Research Center, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran Daryoush Hydarnasab Cancer Research Center, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran Vahid Khori Golestan Cardiovascular research center, Golestan University of Medical Sciences, Gorgan, Iran 2015 10 01 Fumonisins, a family of mycotoxins, are mainly toxic and carcinogenic. The present study was carried out to evaluate fumonisin B1 (FB1) effects on the production of inflammatory cytokines by gastric and colon cell lines. The study was performed on two cell lines under in vitro condition, including gastric epithelial cell line (AGS) and human colon adenocarcinoma cell line (SW742). Lipopolysaccharide  (LPS)  was  used  for  inflammatory  cytokine  induction.  The  culture medium was supplemented with 4.5–72 mg/l of FB1 for 72 h before cell induction. The supernatants were harvested 24 h after the induction and measured for cytokines by using enzyme-linked immunosorbent assay. FB1 induced a dose-dependent increase in the production of tumor necrosis factor-α and interleukin-1β in both AGS and SW742 cell lines. This increase was statistically significant with concentration of FB1 between 9 and 72 mg/l (P < 0.05). FB1 also induced a dose- dependent decrease in interleukin-8 production. This decrease was seen in both cell lines and showed a statistical significance with FB1 concentration (P < 0.05). The results show that FB1 increases inflammatory cytokines production by various gastric and intestinal cells. This effect in the long run can possibly be the basis for the occurrence or development of inflammation and subsequent atrophy in the above-mentioned tissues. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/341 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/341/341