Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 11 2 2012 06 15 147 155 EN Reza Falak Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran AND Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Mojtaba Sankian Immunobiochemistry Laboratory, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Mohsen Tehrani Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sary, Iran Farahzad Jabbari Azad Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Ahmad Abolhasani Dentistry Clinic, Beheshti Teacher Training Center, Mashhad, Iran Abdolreza Varasteh Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran 2015 10 01 Oral allergy syndrome (OAS) is occasionally observed following consumption  of raw fruits  in  allergic adults. Since this  phenomenon  was  commonly  reported  in  Khorasan province of Iran; we intended to check if common  diagnostic tests could be applied for differential diagnosis of OAS to grapes. IgE reactivity of 84 patients with OAS to grape and 34 patients with OAS to other fruits were analyzed by in vivo and in vitro methods, and the results were compared with those of controls. The patients underwent skin prick test (SPT) with common allergic pollen extracts as well as grape extract. The specific IgE  level to grape proteins was determined by an indirect ELISA. The correlation of SPT results with ELISA and western blotting patterns was checked by statistical methods. The results showed a significant correlation of grape SPT diameters with grape specific IgE levels. Furthermore,  a significant association of grape SPT results with IgE immunoreactivity of a 10 kDa  grape protein,  probably lipid transfer protein  (LTP) was prominent. Immunoreactivity of other proteins was linked with mild clinical symptoms. The study showed a significant correlation of grape SPT results with grape total extract, as well as its 10 kDa component’s IgE reactivity. The results suggested that OAS to grape should not be considered as a main criterion in diagnosis of grape allergy and a combination of grape SPT results with evaluation of IgE reactivity to grape 10 kDa allergen should be considered to achieve a more reliable grape allergy diagnosis. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/339 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/339/339