Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 21 1 2022 02 06 81 85 Najmeh Sepahi Allergy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Soheila Alyasin Allergy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran AND Department of Allergy and Clinical Immunology, Namazi Hospital, Shiraz, Iran Zahra Kanannejad Allergy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Hossein Esmaeilzadeh Allergy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran AND Department of Allergy and Clinical Immunology, Namazi Hospital, Shiraz, Iran Farzaneh Mohammadalizadeh Shirazi Allergy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Maryam Babaei Allergy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Shirin Farjadian Allergy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran AND Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran 2021 06 27 2021 10 10 Pollens have been identified as potent inducers of allergic diseases worldwide. Acer velutinum (Persian maple) tree is an important source of allergic pollens in Iran. This study aimed to identify the immunoglobulin E (IgE)-reactive components of A. velutinum pollen extract in patients with maple allergy. We aimed to evaluate its allergenic components; using IgE in the serum of patients with maple allergy. Twenty-two patients with a clinical history of reaction and a positive skin-prick test to maple pollen extract were included in this study. Identification of IgE-binding proteins in A. velutinum pollen extract was performed by immunoblotting using sera from sensitive patients. A protein band with a molecular weight of around 70 kDa was the most IgE-reactive allergen in A. velutinum pollen extract detected by this method. Identification of a protein with a molecular weight of about 70kDa, as the most reactive allergen of A. velutinum pollen extract, can be considered as a potential allergen for designing diagnostic kits or as a target for immunotherapy of allergic patients with maple allergy. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/3274 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/3274/1796