Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 20 5 2021 09 28 584 592 Parisa Rahimzadeh Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran AND Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran Sahar Mortezagholi Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mojgan Ghayedi Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Haideh Namdari Iranian Tissue Bank and Research Center, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran Mitra Rahimzadeh Social Determinants of Health Research Center, Alborz University of Medical Sciences, Karaj, Iran Roobina Boghozian Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Maryam Azimi Immunology Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran Eisa Salehi Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran 2020 07 14 2021 03 17 Mechanisms underlying the systemic lupus erythematosus (SLE) have not yet been elucidated. In this study, we evaluated the balance of T cell subsets in BALB/c mice model of SLE induced; using Con A and polyamines as DNA immunogenicity modifiers. BALB/c mice were immunized subcutaneously with 50 µg extracted DNA from cells cultured in different conditions: splenocytes+ polyamines (group P), splenocytes+ Con A (group A), splenocytes+ polyamines+ Con A (group PA) and splenocytes only (control). Anti-double-stranded DNA –(ds-DNA) antibodies, proteinuria, and antinuclear autoantibodies were assessed by enzyme-linked immunosorbent assay, Bradford method, and immunofluorescence respectively. Transcription factors of different T helper subsets were examined by real-time polymerase chain reaction. The serum level of the anti-dsDNA antibody in group PA was higher than that in the other groups (p>0.05). Antinuclear antibody (ANA) titer increased in groups A and PA. Proteinuria level in group PA was significantly higher than that in the control group (p<0.001). Expression of Foxp3 was decreased in group A (p=0.001). Additionally, the ratios of T-bet/GATA3 and T-bet/Foxp3 were also increased in group A. (p>0.05). Our results revealed an increased ratio of Th1 to Th2 and decreased expression of Foxp3 in group A, but group PA manifested more obvious signs of the disease. These results suggest that other mechanisms rather than disturbance in T cells' balance may involve the development of disease symptoms. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2900 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/2900/1723