Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 9 3 2010 09 15 133 140 Maryam Mahmoudi Department of Nutrition and Biochemistry, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Fereydoon Siassi Department of Nutrition and Biochemistry, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mohammad Jafar Mahmoudi Division of Cardiology, Department of Internal Medicine, Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza Eshraghian Department of Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Amir Hassan Zarnani Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran AND Immunology Research Center, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Nima Rezaei Molecular Immunology Research Center; Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran AND Research Group for Immunodeficiencies, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Mona Hedayat Division of Cardiology, Department of Internal Medicine, Tehran University of Medical Sciences, Tehran, Iran Fazel Shokri Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Ali-Akbar Saboor-Yaraghi Department of Nutrition and Biochemistry, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2015 10 01 Atherosclerosis is a chronic inflammatory disease of the arterial wall characterized by innate and adaptive immune responses to a variety of microbial and self-antigens. Given the crucial role of adaptive immunity in the pathogenesis of atherosclerosis, this study was performed to investigate the proliferative response of peripheral blood mononuclear cells (PBMC) and interleukin (IL)-2 production in patients with coronary artery disease (CAD). In this study, 25 patients with chronic stable CAD and 25 healthy individuals were investigated. The PBMCs were separated and stimulated with phytohaemagglutinin (PHA). MTT assay was performed to measure cell viability and proliferation. IL-2 concentrations in cell culture supernatants were determined by Enzyme-Linked Immunosorbent Assay. PHA-stimulated cells revealed a significantly increased optical density (OD) in both groups of patients (p=0.004) and controls (p https://ijaai.tums.ac.ir/index.php/ijaai/article/view/278 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/278/278