Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 9 2 2010 06 15 59 67 Javad Arasteh Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Zahra Pourpak Immunology Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Masoumeh Ebtekar Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Ali Akbar Pourfathollah Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Zuhair Mohammad Hassan Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Tahereh Farahmandian Department of Obstetric and Gynecology, Tehran University of Medical Sciences, Tehran, Iran Hamid Mahmoudzadeh-Niknam Department of Immunology, Pasteur Institute of Iran, Tehran, Iran 2015 10 01 IL-22 is a member of IL-10 cytokine family which is believed to play an important role in inflammatory responses. IL-22 has similarities with IL-10 including conserved sequences with IL-10. IL-22 receptor is also comprised of two chains known as L-22R1 and L-10R2; supporting the speculation that the two cytokines may have similar effects. The aim of this study was to shed some light on the biological activity of IL-22 upon the cord blood CD4+CD25- T cells. In this research, cord blood T CD4+CD25- cells were cultured in presence of anti CD2/CD3/CD28 coated beads, IL-2 and IL-22 for two weeks at 37 oC and 5% CO2. Flow cytometry analysis showed that IL-22 has no effect upon CD25 and Foxp3 expression. Also, the results indicated that IL-22 is not involved in CD4+ T cell proliferation. Moreover, the results of suppression assay did not show any suppression effect on the cultured T cells. Thus, it seems that umbilical cord blood T cells probably do not express IL-22R1 on their surface. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/270 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/270/270