Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 19 3 2020 06 23 243 252 Alireza Pishgahi Physical Medicine and Rehabilitation Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Rozita Abolhasan Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Seyed Kazem Shakouri Physical Medicine and Rehabilitation Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Mohammad Sadegh Soltani-Zangbar Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran Shahla Dareshiri Physical Medicine and Rehabilitation Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Sepideh Ranjbar Kiyakalayeh Physical Medicine and Rehabilitation Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Amirghasem Khoeilar Stud Road Medical Centre, Dandenong, VIC, Australia Majid Zamani Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Gonabad University of Medical Sciences, Gonabad, Iran Farhad Motavalli Khiavi Medical Biotechnology Research Center, AJA University of Medical Sciences, Tehran, Iran Behzad Pourabbas Kheiraddin Department of Polymer Engineering, Sahand University of Technology, Tabriz, Iran Amir Mehdizadeh Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Mehdi Yousefi Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran 2019 12 15 2020 04 13 Knee osteoarthritis (OA) is one of the common degenerative articular disorders that are related to decreased quality of life. Currently, novel biologic therapeutic approaches are introduced in the literature for OA management. In this study, the clinical efficiency of Dextrose prolotherapy, platelet-rich plasma (PRP) and autologous conditioned serum (ACS) injection on the level of pain and function in Knee OA were compared. A randomized clinical trial was directed on 92 knee OA patients. Patients were randomly divided into three groups: 30 were received dextrose prolotherapy once in a week for three weeks, 30 received autologous PRP for two times with seven days interval, and in the remaining 32 patients 2ml of ACS were injected two times every seven days. Study participants were measured through the Western Ontario and McMaster Universities (WOMAC) score, the visual analogue scale (VAS), at baseline, 1 and 6 months post-intervention. Both ACS and PRP treated patients showed improvement in pain intensity and knee function during 1 and 6 months pursue; however, this progress was more significant in the ACS group. Dextrose prolotherapy showed no substantial changes in pain and function of the affected knee in treated patients. Treatment of Knee OA with ACS and PRP injections are associated with pain reduction and knee function improvement. Not only, ACS therapy is more effective than that of PRP, but also due to its less variability in processing and less reported side effects, it could be considered as a safe and effective non-surgical alternative for OA management. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2639