Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 18 6 2019 11 10 655 663 Kobra Mehdinejadiani Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mojgan Bandehpour Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Ali Hashemi Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mohammad Mehdi Ranjbar Razi Vaccine and Serum Research Institute, Tehran, Iran Sodabeh Taheri Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Seyed Amir Jalali Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Nariman Mosaffa Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2019 04 28 2019 06 23 Acinetobacter baumannii is a Gram-negative bacterium that has recently been identified as a leading nosocomial pathogen. Infections by this pathogen result in significant mortality due to antibiotic resistance. An effective vaccine would help alleviate the burden of disease incurred by this pathogen; however, there are currently no licensed vaccines offering protection against Acinetobacter baumannii infection. In this study, considering the fact that outer membrane protein A is one of the most promising vaccine candidates, we predicted T cell and B cell epitopes on this protein using sequence-based epitope prediction tools and determined whether or not mice immunized with these peptides induce an immune response. We selected consensus epitopes including five peptides in different tools with the highest score. 48 female C5BL/6 SPF injected subcutaneously with the peptides (peptide1 to peptide 5 separately) in 100 μL of the solution and sham groups received adjuvant and PBS alone on the same schedule: on day 0 (primary dose) and two booster doses were administered on days 14 and 28. At the end of time, animals euthanized by Isoflurane, and collected sera for assessment of specific antibodies against each peptide by ELISA (Enzyme-linked immunosorbent assay). Immunization of mice showed one of the novel synthetic peptides (peptide 1 (24-50 amino acids)) elicited immune responses. We conclude to combine theoretical methods of epitope prediction and evaluating the potential of immunogenicity for developing vaccines is important. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2384