Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 8 1 2009 03 15 53 56 Mortazavi Hossein Department of Dermatology, Autoimmune Bullous Diseases Research Center, School of Medicine, Shahdi Majid Department of Dermatology, Autoimmune Bullous Diseases Research Center, School of Medicine, Amirzargar Ali Akbar Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran Iran S.Naraghi Zahra Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran Iran Valikhani Mahin Department of Dermatology, Autoimmune Bullous Diseases Research Center, School of Medicine, Daneshpazhooh Maryam Department of Dermatology, Autoimmune Bullous Diseases Research Center, School of Medicine, Vasheghani-Farahani Amir Department of Dermatology, Autoimmune Bullous Diseases Research Center, School of Medicine, Sedaghat Mojtaba Department of Community Medicine, School of Medicine, Tehran University of Medical Sciences, Tehran Chams-Davatchi Cheyda Department of Dermatology, Autoimmune Bullous Diseases Research Center, School of Medicine, 2015 10 01 Anti-desmoglein 3 and 1 autoantibodies are involved in the pathogenesis of pemphigus diseases. Our objective was to assess the value of ELISA in the diagnosis of pemphigus and its correlation with the severity of pemphigus vulgaris. Based on clinical presentation and histopathologic confirmation for the diagnosis of the pemphigus, 38 patients took part in the study. Sera of the patients were tested by desmoglein 1 and desmoglein 3 ELISA. Also, direct immunofluorescence was performed for all patients which revealed positive results in 36 patients (94.7%). ELISA was positive in 37 of 38 pemphigus patients (Sensitivity: 97.3%). The relationship between desmoglein 1 index values and skin severity was statistically significant (p https://ijaai.tums.ac.ir/index.php/ijaai/article/view/234 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/234/234