Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 17 5 2018 10 20 464 474 EN Hamed Mohammadi Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran AND Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran Farhad Babaie Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Maryam Hemmatzadeh Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Gholamreza Azizi Non-Communicable Diseases Research Center, Alborz University of Medical Sciences, Karaj, Iran AND Department of Laboratory Medicine, Imam Hassan Mojtaba Hospital, Alborz University of Medical Sciences, Karaj, Iran Mehrzad Hajaliloo Connective Tissue Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Ali Asghar Ebrahimi Connective Tissue Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Tohid Kazemi Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Mehdi Yousefi Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran AND Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Alireza Rezaiemanesh Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran Elham Safarzadeh Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Elham Baghbani Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Jafar Majidi Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Behzad Baradaran Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran 2018 08 19 2018 09 05 Ankylosing spondylitis (AS), an autoinflammatory disease, has been associated with impaired Endoplasmic reticulum aminopeptidase (ERAP) 1 activity, which is involved in priming antigenic peptides. The purpose of this study was to evaluate if the genetic variant of ERAP1 gene could impress the inflammation status of the AS patients. For genotyping, 140 AS cases and 140 healthy controls were enrolled. After isolation of peripheral blood mononuclear cells (PBMCs) and DNA extraction, all the subjects were genotyped for rs27044 polymorphism using SSP-PCR assay. Total RNA of PBMCs was isolated, cDNA was synthesized, and quantitative analyses of mRNA expression of cytokines were performed via Real-time PCR using the SYBR Green Gene Expression MasterMix. To measure the concentration of cytokines in serum of subjects, Enzyme-linked immunosorbent assay (ELISA) was used. It was observed that the G allele of rs27044 polymorphism was significantly prevalent in AS patients. Moreover, the GG genotype and the GG+GC dominant model had significantly different distribution between study groups. There was a significant overexpression of mRNAs of IL-17A, IL-6, IL-33, TNF-α, and IFN-γ, while IL-10 was significantly downregulated in AS patients. The ELISA results were in line with that of the gene expression analysis. No significant differences in mRNA expression and concentration of cytokine were identified among AS patients with three genotypes for rs27044 SNP. This study replicated the association of polymorphisms in ERAP1 gene with the risk of AS in a population from Iranian. However, it did not directly determine the inflammatory profile of the AS patients. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2119 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/2119/1371