Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 7 2 2008 06 15 61 68 Gholamhossein Hassanshahi Abdollah Jafarzadeh Alan James Dickson 2015 10 01 Stromal derived factor-a (SDF-1-a) is a CXC chemokine which has been demonstrated as a recruitment factor for leukocytes to the site of inflammation, infection, injury and following stress. This chemokine has been shown to be expressed by liver cells and in liver diseases. Hence, the aim of this study was to examine the expression of SDF-1 by hepatocytes in responses to the stress imposed during isolation by collagenase perfusion and under heat shock stimulation. In this study hepatocytes (2-5 x 106) were isolated from male Sprague Dawley rat liver and cultured in plates that were pre-coated with collagen Type-I matrix. The western and northern blotting analysis were employed to detect SDF-1 at protein and mRNA levels in isolated and cultured hepatocytes in response to isolation and heat shock stresses. The SDF-1 is expressed by isolated rat hepatocytes immediately after isolation and early culture and decreased with time. SDF-1 protein was highly expressed in freshly isolated cells and decreased by time (27h) (P https://ijaai.tums.ac.ir/index.php/ijaai/article/view/201 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/201/201