Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 5 2 2006 06 15 75 78 "Fatemeh Ghaffarifar Abdolhossein Dalimi Asl Zohreh Sharifi Sakineh Ghasemi Kavos Solhjoo Shahla Roodbar Mohammadi " 2015 10 01 Toxoplasmosis is a protozoal infection caused by Toxoplasma gondii. Toxoplasmosis produce severe damage in patients who are immunosuppresed. In those who are immunosupressed, latent infection can be reactivated resulting in acute disseminating disease. Betamethasone is a synthetic glycocorticoid, used as an anti-inflamatory and immunosuppressant in a wide variety of disorders.The aim of this study was evaluation of betamethasone as an immunosuppressor drug on infected cells by Toxoplasma gondii. In this study, at first HeLa cells were grown in 24 well culture plates in culture medium .When confluent monolayer was obtained, we compared 6 groups to evaluate the effect of betamethasone as a corticosteroid drug (two concentrations 4 and 40μg/ml) and the effect of IFN-γ (100 IU/ml ) on growth, replication and Nitric Oxide (NO) production. The results showed, that high number of plaques were seen in group with 40 g/ml of betamethasone and the lowest number of plaques were seen in group with 100 IU of IFN-. The difference between plaque number in control and groups treated with IFN- and betamethasone was significant (P https://ijaai.tums.ac.ir/index.php/ijaai/article/view/137 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/137/137