Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 16 2 2017 05 07 120 126 EN Meisam Barati Student Research Committee, School of Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran Mehdi Yousefi Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Mehrangiz Ebrahimi-Mameghani Nutrition Research Center, Department of Community Nutrition, School of Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran Hamed Mohammadi Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Balal Brazvan Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz. Iran Hamid Nickho Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Mehdi Fouladi Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran Mohammad Mohammadi Department of Nutrition, Faculty of Health, Iran University of Medical Sciences, Tehran, Iran 2016 07 22 2016 10 09 Oryzatensin (ORZ) can reduce potentially IFN-γ secretion by natural killer (NK) cells. Therefore, current study was designed to evaluate the effects of ORZ treatment on peripheral blood mono-nuclear cells (PBMCs) cytokine secretion, proliferation and also to evaluate vascular endothelial growth factor (VEGF) and Matrix Metalloproteinase 9 (MMP-9) expression in HEP-G2 cell line after culture with ORZ-stimulated PBMCs. In this ex-vivo study, PBMCs from apparently healthy male volunteers (n=25) aged 20-30 were isolated by ficoll density gradient. Tetrazolium colorimetric test (MTT assay), ELISA test and real time PCR were performed to evaluate PBMCs proliferation, PBMCs cytokine secretion and the genes expression accordingly. The results of MTT assay showed that ORZ significantly stimulated proliferation of the isolated PBMCs. The results also indicated that ORZ treatment significantly decrease and increase IFN-γ and IL-4 secretion by isolated PBMCs, respectively. Also, VEGF and MMP-9 expression significantly increased in HEP-G2 cells after culture with ORZstimulated PBMCs. The previous studies have introduced ORZ-like peptide for pharmacological purpose and in this study we get to the conclusion that the administration of this peptide may change the immune system response and sensitize target populations to cancer. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1008 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/1008/726