Evaluation of ERAP1 Gene Single Nucleotide Polymorphism in Impressing the Inflammatory Cytokine Profile of Ankylosing Spondylitis Patients

  • Hamed Mohammadi Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran AND Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
  • Farhad Babaie Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  • Maryam Hemmatzadeh Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  • Gholamreza Azizi Non-Communicable Diseases Research Center, Alborz University of Medical Sciences, Karaj, Iran AND Department of Laboratory Medicine, Imam Hassan Mojtaba Hospital, Alborz University of Medical Sciences, Karaj, Iran
  • Mehrzad Hajaliloo Connective Tissue Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Ali Asghar Ebrahimi Connective Tissue Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Tohid Kazemi Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  • Mehdi Yousefi Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran AND Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Alireza Rezaiemanesh Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
  • Elham Safarzadeh Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  • Elham Baghbani Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Jafar Majidi Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  • Behzad Baradaran Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Keywords: Ankylosing spondylitis, Cytokine, Endoplasmic reticulum aminopeptidase, Gene expression, Polymorphisms

Abstract

Ankylosing spondylitis (AS), an autoinflammatory disease, has been associated with impaired Endoplasmic reticulum aminopeptidase (ERAP) 1 activity, which is involved in priming antigenic peptides. The purpose of this study was to evaluate if the genetic variant of ERAP1 gene could impress the inflammation status of the AS patients. For genotyping, 140 AS cases and 140 healthy controls were enrolled. After isolation of peripheral blood mononuclear cells (PBMCs) and DNA extraction, all the subjects were genotyped for rs27044 polymorphism using SSP-PCR assay. Total RNA of PBMCs was isolated, cDNA was synthesized, and quantitative analyses of mRNA expression of cytokines were performed via Real-time PCR using the SYBR Green Gene Expression MasterMix. To measure the concentration of cytokines in serum of subjects, Enzyme-linked immunosorbent assay (ELISA) was used. It was observed that the G allele of rs27044 polymorphism was significantly prevalent in AS patients. Moreover, the GG genotype and the GG+GC dominant model had significantly different distribution between study groups. There was a significant overexpression of mRNAs of IL-17A, IL-6, IL-33, TNF-α, and IFN-γ, while IL-10 was significantly downregulated in AS patients. The ELISA results were in line with that of the gene expression analysis. No significant differences in mRNA expression and concentration of cytokine were identified among AS patients with three genotypes for rs27044 SNP. This study replicated the association of polymorphisms in ERAP1 gene with the risk of AS in a population from Iranian. However, it did not directly determine the inflammatory profile of the AS patients.

References

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Published
2018-10-20
How to Cite
1.
Mohammadi H, Babaie F, Hemmatzadeh M, Azizi G, Hajaliloo M, Ebrahimi AA, Kazemi T, Yousefi M, Rezaiemanesh A, Safarzadeh E, Baghbani E, Majidi J, Baradaran B. Evaluation of ERAP1 Gene Single Nucleotide Polymorphism in Impressing the Inflammatory Cytokine Profile of Ankylosing Spondylitis Patients. ijaai. 17(5):464-7.
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