Effect of Progesterone on Expression of MMP7 and MMP13 in Lungs of Female Mice

  • Elahe Izadi International Campus, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Fateme Vafashoar Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Paria Jorbozedar Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Pendar Safari Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Mohamad Ali Assarehzadegan Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Hadi Poormoghim Scleroderma Study Group, Firuzgar Hospital, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Jalil Kuhpayezadeh Community Medicine, Preventive Medicine and Public Health Research Center, Iran University of Medical Sciences, Tehran, Iran
  • Nazanin Mojtabavi Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Keywords: Collagenase, Extracellular matrix, Lung diseases, Matrilysin, Matrix metalloproteinases, Progesterone

Abstract

Gender medicine is a new era of science which focuses on the impact of sex hormones and gender on normal physiology, pathobiology and clinical features of diseases. In this study we investigated the impact of pregnancy doses of progesterone hormone on the expression of a couple of matrix metalloproteinase (MMPs), which are known to be involved in tissue remodeling of lungs in health and disease, namely MMP7 and 13. Pregnancy maintenance dose of progesterone was administered to female BALB/c mice for 21 and 28 days, the control group received PBS for the same days. After removal of the lungs and RNA extraction, quantitative real-time PCR was done using specific primers for MMP7 and MMP13. We found that progesterone can slightly (not significantly) decrease the expression of MMP13 but had no effect on MMP7. Our results shows that progesterone has minimal effect on the expression of matrix metalloproteinase7 and matrix metalloproteinase 13, but it may still have an effect on corresponding tissue inhibitor of matrix metalloproteinases (TIMPs) or other components of the Extracellular matrix  which remains to be elucidated. Also, the effect of progesterone on these MMPs can be further studied in a fibrosis model.

Author Biographies

Elahe Izadi, International Campus, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Iran University of Medical Sciences
Fateme Vafashoar, Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Iran University of Medical Sciences
Paria Jorbozedar, Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Iran University of Medical Sciences
Pendar Safari, Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Iran University of Medical Sciences
Mohamad Ali Assarehzadegan, Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Iran University of Medical Sciences
Hadi Poormoghim, Scleroderma Study Group, Firuzgar Hospital, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Iran University of Medical Sciences
Jalil Kuhpayezadeh, Community Medicine, Preventive Medicine and Public Health Research Center, Iran University of Medical Sciences, Tehran, Iran
Iran University of Medical Sciences
Nazanin Mojtabavi, Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Iran University of Medical Sciences

References

1. Kocurek, E.G. and A.R. Hemnes, Women's Health and Lung Development and Disease. Obstet Gynecol Clin North Am, 2016. 43(2): p. 307-23.2. Carey, M.A., et al., It's all about sex: male-female differences in lung development and disease. Trends in endocrinology and metabolism: TEM, 2007. 18(8): p. 308-313.3. Townsend, E.A., V.M. Miller, and Y.S. Prakash, Sex differences and sex steroids in lung health and disease. Endocr Rev, 2012. 33(1): p. 1-47.4. Tam, A., et al., The role of female hormones on lung function in chronic lung diseases. BMC Womens Health, 2011. 11: p. 24.5. Schatz, M., S. Clark, and C.A. Camargo, Jr., Sex differences in the presentation and course of asthma hospitalizations. Chest, 2006. 129(1): p. 50-5.6. Almqvist, C., M. Worm, and B. Leynaert, Impact of gender on asthma in childhood and adolescence: a GA2LEN review. Allergy, 2008. 63(1): p. 47-57.7. Becklake, M. and F. Kauffmann, Gender differences in airway behaviour over the human life span. Thorax, 1999. 54(12): p. 1119-1138.8. Fu, L., et al., Natural progression of childhood asthma symptoms and strong influence of sex and puberty. Ann Am Thorac Soc, 2014. 11(6): p. 939-44.9. Ligeiro de Oliveira, A.P., et al., Regulation of allergic lung inflammation in rats: interaction between estradiol and corticosterone. Neuroimmunomodulation, 2004. 11(1): p. 20-7.10. Vink, N.M., et al., Gender differences in asthma development and remission during transition through puberty: the TRacking Adolescents' Individual Lives Survey (TRAILS) study. J Allergy Clin Immunol, 2010. 126(3): p. 498-504.e1-6.11. Card, J.W. and D.C. Zeldin, Hormonal Influences on Lung Function and Response to Environmental Agents: Lessons from Animal Models of Respiratory Disease. Proceedings of the American Thoracic Society, 2009. 6(7): p. 588-595.12. Parks, W.C. and S.D. Shapiro, Matrix metalloproteinases in lung biology. Respiratory Research, 2000. 2(1): p. 3.13. Loffek, S., O. Schilling, and C.W. Franzke, Series "matrix metalloproteinases in lung health and disease": Biological role of matrix metalloproteinases: a critical balance. Eur Respir J, 2011. 38(1): p. 191-208.14. Demedts, I.K., et al., Matrix metalloproteinases in asthma and COPD. Curr Opin Pharmacol, 2005. 5(3): p. 257-63.15. Fanjul-Fernandez, M., et al., Matrix metalloproteinases: evolution, gene regulation and functional analysis in mouse models. Biochim Biophys Acta, 2010. 1803(1): p. 3-19.16. Yan, C. and D.D. Boyd, Regulation of matrix metalloproteinase gene expression. J Cell Physiol, 2007. 211(1): p. 19-26.17. Sathish, V., Y.N. Martin, and Y.S. Prakash, Sex steroid signaling: implications for lung diseases. Pharmacol Ther, 2015. 150: p. 94-108.18. Matsubara, S., et al., Estrogen determines sex differences in airway responsiveness after allergen exposure. Am J Respir Cell Mol Biol, 2008. 38(5): p. 501-8.19. Clutterbuck, A.L., et al., Targeting matrix metalloproteinases in inflammatory conditions. Curr Drug Targets, 2009. 10(12): p. 1245-54.20. Watelet, J.B., et al., Matrix metalloproteinases MMP-7, MMP-9 and their tissue inhibitor TIMP-1: expression in chronic sinusitis vs nasal polyposis. Allergy, 2004. 59(1): p. 54-60.21. Goswami, S., et al., Divergent functions for airway epithelial matrix metalloproteinase 7 and retinoic acid in experimental asthma. Nat Immunol, 2009. 10(5): p. 496-503.22. Clarke, D.L., et al., Matrix regulation of idiopathic pulmonary fibrosis: the role of enzymes. Fibrogenesis & Tissue Repair, 2013. 6(1): p. 20.23. Wadsworth, S.J., et al., IL-13 and TH2 cytokine exposure triggers matrix metalloproteinase 7-mediated Fas ligand cleavage from bronchial epithelial cells. J Allergy Clin Immunol, 2010. 126(2): p. 366-74, 374.e1-8.24. Dunsmore, S.E., et al., Matrilysin expression and function in airway epithelium. The Journal of Clinical Investigation, 1998. 102(7): p. 1321-1331.25. Mori, S., et al., Expression and Roles of MMP-2, MMP-9, MMP-13, TIMP-1, and TIMP-2 in Allergic Nasal Mucosa. Allergy, Asthma & Immunology Research, 2012. 4(4): p. 231-239.26. Nkyimbeng, T., et al., Pivotal Role of Matrix Metalloproteinase 13 in Extracellular Matrix Turnover in Idiopathic Pulmonary Fibrosis. PLOS ONE, 2013. 8(9): p. e73279.27. Cvetkovski, F., et al., MMP-13 is a Critical Driver of Lung Destruction in a Viral Exacerbation Model of COPD, in C98. AIRWAY REMODELING IN COPD AND ASTHMA. 2016. p. A6176-A6176.28. Solı́s-Herruzo, J.A., et al., Interleukin-6 Increases Rat Metalloproteinase-13 Gene Expression through Stimulation of Activator Protein 1 Transcription Factor in Cultured Fibroblasts. Journal of Biological Chemistry, 1999. 274(43): p. 30919-30926.29. Mengshol, J.A., M.P. Vincenti, and C.E. Brinckerhoff, IL-1 induces collagenase-3 (MMP-13) promoter activity in stably transfected chondrocytic cells: requirement for Runx-2 and activation by p38 MAPK and JNK pathways. Nucleic Acids Research, 2001. 29(21): p. 4361-4372.30. Moriya, C., et al., Expression of matrix metalloproteinase-13 is controlled by IL-13 via PI3K/Akt3 and PKC-delta in normal human dermal fibroblasts. J Invest Dermatol, 2011. 131(3): p. 655-61.31. Leivonen, S.K., et al., Smad3 mediates transforming growth factor-beta-induced collagenase-3 (matrix metalloproteinase-13) expression in human gingival fibroblasts. Evidence for cross-talk between Smad3 and p38 signaling pathways. J Biol Chem, 2002. 277(48): p. 46338-46.32. Leeman, M.F., S. Curran, and G.I. Murray, The structure, regulation, and function of human matrix metalloproteinase-13. Crit Rev Biochem Mol Biol, 2002. 37(3): p. 149-66.33. Chakrabarti, S. and K.D. Patel, Matrix metalloproteinase-2 (MMP-2) and MMP-9 in pulmonary pathology. Exp Lung Res, 2005. 31(6): p. 599-621.34. Potier, M., et al., Expression and regulation of estrogen receptors in mesangial cells: influence on matrix metalloproteinase-9. J Am Soc Nephrol, 2001. 12(2): p. 241-51.35. Elliot, S., et al., Subtype Specific Estrogen Receptor Action Protects Against changes in MMP-2 Activation in Mouse Retinal Pigmented Epithelial Cells. Experimental eye research, 2008. 86(4): p. 653-660.36. Pirila, E., et al., Wound healing in ovariectomized rats: effects of chemically modified tetracycline (CMT-8) and estrogen on matrix metalloproteinases -8, -13 and type I collagen expression. Curr Med Chem, 2001. 8(3): p. 281-94.

Published
2018-10-20
How to Cite
1.
Izadi E, Vafashoar F, Jorbozedar P, Safari P, Assarehzadegan MA, Poormoghim H, Kuhpayezadeh J, Mojtabavi N. Effect of Progesterone on Expression of MMP7 and MMP13 in Lungs of Female Mice. ijaai. 17(5):485-9.
Section
Brief Communication