Genetic Variation in Intergenic and Exonic miRNA Sequence and Risk of Multiple Sclerosis in the Isfahan Patients

  • Zeynab Golshani Department of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran AND Department of Biology, Fars Science and Research Branch, Islamic Azad University, Shiraz, Iran
  • Zohreh Hojati Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran
  • Ali Sharifzadeh Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
  • Vahid Shaygannejad Isfahan Neurosciences Research Center, Department of Neurology, Alzahra Hospital, Isfahan University of Medical Sciences, Isfahan, Iran
  • Mojtaba Jafarinia Department of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran
Keywords: Genetic variation, MiRNA, Multiple sclerosis, T ARMS PCR

Abstract

MicroRNAs (miRNAs), have been documented to perform a key role in the pathogenesis of multiple sclerosis (MS), a chronic inflammatory and autoimmune disease. Recent studies have shown that single nucleotide polymorphism in the sequence of the miRNA may change their production and expression which can lead to miRNA dysfunction and pathogenicity. Some studies have reported the relationship between miRNA polymorphism and the increased risk of autoimmune disease. This study was conducted to investigate the association between mir155 rs767649, mir196a2 rs11614913 and mir23a rs3745453 polymorphism and the risk of multiple sclerosis in the Iranian MS patients in Isfahan. A population of 80 patients and the same number control were selected. After DNA extraction, genotyping was performed through tetra amplification refractory mutation system-PCR method (T ARMS PCR). The frequencies of TT, TC and CC genotypes of mir23a were 46, 35 and 20% in MS patients and 42, 14 and 24 in healthy subjects respectively. These results showed that individuals carrying the genotypes of rs3745453 TC had a 2.3-fold increased risk of MS (OR=2.3, p=0.048). There was no significant difference between genotypes and allele frequency of mir155 and mir196a2 in patients and healthy controls (p>0.05). Our findings specified that CT heterozygosity in mir23a gene significantly related with risk of MS. Unlike mir155 and mir196a2, mir23a rs3745453 may have contributed to the etiology of MS in Isfahan patients. However, extensive studies are required to gain more reliable and authentic results.

Author Biographies

Zohreh Hojati, Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran
Isfahan university
Ali Sharifzadeh, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Islamic azaduniversity of shahrekord
Vahid Shaygannejad, Isfahan Neurosciences Research Center, Department of Neurology, Alzahra Hospital, Isfahan University of Medical Sciences, Isfahan, Iran
medical science of Isfahan university
Mojtaba Jafarinia, Department of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran
islamic azad universityof marvdasht,shiraz

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Published
2018-10-14
How to Cite
1.
Golshani Z, Hojati Z, Sharifzadeh A, Shaygannejad V, Jafarinia M. Genetic Variation in Intergenic and Exonic miRNA Sequence and Risk of Multiple Sclerosis in the Isfahan Patients. ijaai. 17(5):1-477.
Section
Brief Communication