Iranian Journal of Allergy, Asthma and Immunology 2017. 16(5):378-385.

Investigating the Association of ADAM33 Single Nucleotide Polymorphisms (SNPs) with Susceptibility to Allergic Asthma in Azerbaijan Population of Iran: A Case-control Study
Ilghar Zeinaly, Mahnaz Sadeghi-Shabestrai, Zohreh Babaloo, Alireza Razavi, Mohammad Sajay-Asbaghi, Saeed Sadigh-Eteghad, Tohid Kazemi

Abstract


Asthma, affecting a growing number of populations, is a clinical condition with complex cellular and genetic factors. Single nucleotide polymorphisms (SNPs) in gene coding for molecules, which play major roles in the immunopathogenesis of asthma have been considered recently as genetic predisposing factors this disease. Possible association between two SNPs in a disintegrin and metalloprotease 33 (ADAM33), which participates in airway remodeling, and susceptibility to asthma was studied in this study. 190 patients with asthma and 180 healthy controls were enrolled in this case-control study. Using conventional PCR method, specific bands were amplified and the frequency of genotypes of T1 (rs2280091) and V4 (rs2787094) ADAM33 SNPs were determined by digestion with NcoI and PstI, respectively. The results showed that the frequency of genotypes of T1 and V4 were not significantly different between patients and controls (p=0.54 and p=0.85, respectively). On the other hand, no significant differences were seen in allele frequency of both T1 and V4 SNPs (p=0.15 and p=0.47, respectively). In agreement with some other studies in different populations, our results showed no association between frequency of genotype or alleles of both T1 and V4 SNPs in ADAM33 gene and predisposition to asthma in Azerbaijan population of Iran. Genetic differences in different ethnic groups might be involved in such inconsistent results. More studies in populations with larger number of patients and healthy individuals are needed for concluding remarks for involvement of ADAM33 SNPs in asthma. 


Keywords


Asthma; ADAM33; Single nucleotide polymorphism (SNP)

Full Text:

PDF

References


1.            Borish L. The immunology of asthma: Asthma phenotypes and their implications for personalized treatment. Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology. 2016;117(2):108-14.

2.            Ober C, Hoffjan S. Asthma genetics 2006: the long and winding road to gene discovery. Genes and immunity. 2006;7(2):95-100.

3.            Giebeler N, Zigrino P. A Disintegrin and Metalloprotease (ADAM): Historical Overview of Their Functions. Toxins. 2016;8(4):122.

4.            Umland SP, Garlisi CG, Shah H, Wan Y, Zou J, Devito KE, et al. Human ADAM33 messenger RNA expression profile and post-transcriptional regulation. American journal of respiratory cell and molecular biology. 2003;29(5):571-82.

5.            Foley SC, Mogas AK, Olivenstein R, Fiset PO, Chakir J, Bourbeau J, et al. Increased expression of ADAM33 and ADAM8 with disease progression in asthma. The Journal of allergy and clinical immunology. 2007;119(4):863-71.

6.            Van Eerdewegh P, Little RD, Dupuis J, Del Mastro RG, Falls K, Simon J, et al. Association of the ADAM33 gene with asthma and bronchial hyperresponsiveness. Nature. 2002;418(6896):426-30.

7.            Liang S, Wei X, Gong C, Wei J, Chen Z, Deng J. A disintegrin and metalloprotease 33 (ADAM33) gene polymorphisms and the risk of asthma: a meta-analysis. Human immunology. 2013;74(5):648-57.

8.            Tajik N, Kazemi T, Delbandi A, Ghods A, Salek Moghaddam A. The Predictive Value of HLA-DR Matching and Cytokine Gene Polymorphisms in Renal Allograft Acute Rejection: a Living-unrelated Donor (LURD) Study. Iranian journal of immunology : IJI. 2006;3(4):150-6.

9.            Su D, Zhang X, Sui H, Lu F, Jin L, Zhang J. Association of ADAM33 gene polymorphisms with adult allergic asthma and rhinitis in a Chinese Han population. BMC medical genetics. 2008;9:82.

10.          Raby BA, Silverman EK, Kwiatkowski DJ, Lange C, Lazarus R, Weiss ST. ADAM33 polymorphisms and phenotype associations in childhood asthma. The Journal of allergy and clinical immunology. 2004;113(6):1071-8.

11.          Lind DL, Choudhry S, Ung N, Ziv E, Avila PC, Salari K, et al. ADAM33 is not associated with asthma in Puerto Rican or Mexican populations. American journal of respiratory and critical care medicine. 2003;168(11):1312-6.

12.          Lee JH, Park HS, Park SW, Jang AS, Uh ST, Rhim T, et al. ADAM33 polymorphism: association with bronchial hyper-responsiveness in Korean asthmatics. Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology. 2004;34(6):860-5.

13.          Blakey J, Halapi E, Bjornsdottir US, Wheatley A, Kristinsson S, Upmanyu R, et al. Contribution of ADAM33 polymorphisms to the population risk of asthma. Thorax. 2005;60(4):274-6.

14.          Werner M, Herbon N, Gohlke H, Altmuller J, Knapp M, Heinrich J, et al. Asthma is associated with single-nucleotide polymorphisms in ADAM33. Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology. 2004;34(1):26-31.

15.          Schedel M, Depner M, Schoen C, Weiland SK, Vogelberg C, Niggemann B, et al. The role of polymorphisms in ADAM33, a disintegrin and metalloprotease 33, in childhood asthma and lung function in two German populations. Respiratory research. 2006;7:91.

16.          Wang P, Liu QJ, Li JS, Li HC, Wei CH, Guo CH, et al. Lack of association between ADAM33 gene and asthma in a Chinese population. International journal of immunogenetics. 2006;33(4):303-6.

17.          Kedda MA, Duffy DL, Bradley B, O'Hehir RE, Thompson PJ. ADAM33 haplotypes are associated with asthma in a large Australian population. European journal of human genetics : EJHG. 2006;14(9):1027-36.

18.          Thongngarm T, Jameekornrak A, Limwongse C, Sangasapaviliya A, Jirapongsananuruk O, Assawamakin A, et al. Association between ADAM33 polymorphisms and asthma in a Thai population. Asian Pacific journal of allergy and immunology / launched by the Allergy and Immunology Society of Thailand. 2008;26(4):205-11.

19.          Vergara CI, Acevedo N, Jimenez S, Martinez B, Mercado D, Gusmao L, et al. A Six-SNP haplotype of ADAM33 is associated with asthma in a population of Cartagena, Colombia. International archives of allergy and immunology. 2010;152(1):32-40.

20.          Karimi MR, Faridhosseini R, Abbaszadegan MR, Azad FJ, Shirkani A, Riyahi A, et al. Association of ADAM33 gene polymorphisms with allergic asthma. Iranian journal of basic medical sciences. 2014;17(9):716-21.

21.          Wang J, Simayi M, Wushouer Q, Xia Y, He Y, Yan F, et al. Association between polymorphisms in ADAM33, CD14, and TLR4 with asthma in the Uygur population in China. Genetics and molecular research : GMR. 2014;13(2):4680-90.

22.          Howard TD, Postma DS, Jongepier H, Moore WC, Koppelman GH, Zheng SL, et al. Association of a disintegrin and metalloprotease 33 (ADAM33) gene with asthma in ethnically diverse populations. The Journal of allergy and clinical immunology. 2003;112(4):717-22.

23.          Awasthi S, Tripathi P, Ganesh S, Husain N. Association of ADAM33 gene polymorphisms with asthma in Indian children. Journal of human genetics. 2011;56(3):188-95.

24.          Tripathi P, Awasthi S, Prasad R, Husain N, Ganesh S. Association of ADAM33 gene polymorphisms with adult-onset asthma and its severity in an Indian adult population. Journal of genetics. 2011;90(2):265-73.


Refbacks

  • There are currently no refbacks.


Creative Commons Attribution-NonCommercial 3.0

This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.